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To further investigate whether WRN acetylation could affect cellular response to MMC treatment, we generated three stable transfectant cell lines.
To further determine whether RAC1 suppression is involved in the suppression of cell migration upon gefitinib treatment, we generated PC-9 cells overexpressing constitutive active forms of RAC1, RAC1G12V or RAC1Q61L.
To validate the role of NRF2 in mediating cellular survival in response to arsenic treatment, we generated tumor cells (A549 lung carcinoma) that were deficient for NRF2 expression using shRNAs (see Methods).
In order to test phosphorylation status of p38 and p53 with in vivo NSC-87877 treatment, we generated xenografts with SH-SY5Y luciferase-tagged cells as above and grew the tumors for 14 days.
Using the subset of genes found to be differentially expressed by letrozole treatment, we generated two sets of coexpressed gene pairs, those that occur in the untreated tumor samples and those that occur in the letrozole-treated tumor samples.
To capture dynamic coexpression changes throughout the course of treatment, we generated three networks: one representing the untreated tumors, one representing early changes following 14 days on letrozole, and one representing late changes following 90 days on letrozole.
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To compare the capacity of different ARR1 versions to promote cytokinin responses in the absence of cytokinin treatments, we generated plants expressing wild-type ARR1 and the phosphomimic ARR1D94E in the arr1-1 background.
To assess the contributions of GLP-1 and REG3B expression on the β-cell protection from low-dose STZ treatments, we generated and tested an AAV9 vector that carries only Reg3b cDNA.
To analyse whether such HIF1 target genes were affected by our treatments we generated a list of HIF1target genes (list contained two gene sets from the GSEA C3 TFT database and the "HIF1_Targets" gene set (C2) that was generated after Semenza (2001) [ 28]).
To discern how steady-state transcript accumulation changes in response to multiple environmental stress treatments, we generated a total of 45,784 ESTs from leaf and berry tissues from vines subjected to abiotic stresses (e.g., salinity, cold, heat, water deficit, and anoxia).
To assess whether tissue cells or cells derived from the hematopoietic system are affected by ONX 0914 treatment (Fig 2A) we generated bone marrow chimeras.
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