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The cells are kept in the presence of cycloheximide from before PEG treatment to block any de novo FP synthesis.
One example already in practice is the high-protein diet suggested during digoxin treatment to block inactivation of digoxin by E. lenta (Haiser et al., 2013).
We selected pioglitazone as the postnatal treatment to block or ease the impairments induced by LPS because although it was designed as an anti-diabetic drug (insulin effect), it also exerts anti-inflammatory effects.
To measure kinetics of cell death without the presence of mitotic slippage, we knocked down Cdc20 by siRNA treatment to block cyclin B1 proteolysis.
The ability of EDTA treatment to block RSV-induced DC maturation suggests that binding of viral particles to C-type lectins on the cell surface is required for DC maturation.
To test this, we exposed BHK-21 cells to wild-type MuHV-4 virions for 4 h, with or without concurrent cycloheximide treatment to block protein synthesis, then stained the cells for ORFs 75a, 75b and 75c.
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Because understanding the modes of entry into the organism of viruses transmitted through sexual intercourse is a necessary prerequisite to the design of treatments to block those infections, in vitro modeling of the transmission is essential.
These insights can be used to model hepatoblastoma development, and to design rational treatments to block cancer progression and recover hepatic function (Goessling et al., 2007).
An understanding of the mechanisms involved in cartilage degradation that occur in rheumatoid arthritis and osteoarthritis is essential for the development of treatments to block disease progression.
In June of each year prior to Alliaria seed dispersal we erect a barrier at the border of the two treatments to block seed dispersal from the ambient into the Alliaria removal treatment.
See related editorial by Katiyar and Raman, http://arthritis-research.com/content/13/4/121 Osteoarthritis (OA) is a multifactorial degenerative joint disease which involves articular cartilage matrix destruction and for which there is no cure and no useful treatments to block disease progression.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com