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Interestingly, p62 degradation and LC3-II accumulation gradually returned to normal levels by 36 h post treatment (statistical data shown in Supplementary information, Figure S8).
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To gain an understanding of the magnitude of treatment effects, statistical data from each study were converted into individual effect sizes (Hedges' g) using the Comprehensive Meta-Analysis version 2 software program (http://www.meta-analysis.com).
The data shown represent an average of 5 to 6 independent measurements per treatment and related statistical data are provided in additional file 2. Rapid response curves of photosynthesis versus irradiance were also measured.
The innovative part of the present research lies in the sum of complementary analytical techniques applied, in the particular pre-treatment of soil samples and in the statistical data treatment.
The statistical data treatment and the stress stability test proposed in this work are suitable to make a reliable stability prediction of different formulations with the same drug, within its pharmaceutical development.
Subsequently, we applied quantitative chemical analysis by energy-dispersive x-ray fluorescence spectrometry (EDXRF) and statistical data treatment on a significant number of those samples.
Statistical data treatment was performed using the BD Image Data Explorer software (BD Biosciences).
The statistical data treatment was performed using The Unscrambler X 10.1 (CAMO Software AS, Oslo, Norway).
For all other experiments, at least five samples per treatment group entered the statistical data analysis.
Graph Pad Prism software version 3.02 (GraphPad Software Inc., CA) was used for statistical data treatment.
FP, PL, MC-B, LG: conception, analysis of the data, drafting of the manuscript; FP: experimental work and data acquisition; FP, LG, MC B: statistical data treatment.
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