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Figure 4 X-ray diffraction pattern of precipitates from the AC treatment collected after 7 days.
The primary outcome was the number of intra-oral radiographs taken per course of treatment collected from a central fee claims database.
Cells were detached after 72 h by standard trypsin/EDTA treatment, collected by centrifugation at 500×g 4°CC, 5 min), washed twice in PBS supplemented with 5% FCS (4°C), and resuspended in PBS plus 5% FCS (4°C) for subsequent fluorescent cell enumeration using the FL1 channel (530/30 nm filter) of a FACSCalibur cytometer (Becton Dickinson, Franklin Lakes, NJ, USA).
This paper makes use of additional information on shopkeepers' knowledge of malaria and its treatment, collected during the same survey.
Control and treated cells were trypsinized at 24 or 48 hours after treatment, collected in PBS and fixed on ice, followed by washing with 70% cold ethanol.
Psychopathological characteristics included history of depression or psychological disorder requiring treatment (collected for all questionnaires), current depression or psychological disorder requiring treatment, psychotropic drug use (collected at the fourth, sixth and seventh questionnaires), depression, anxiety or tears at menopause.
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Pair-wise statistical analyses were performed using Student's t-tests between sets of biological replicates from different treatments collected during the same time period only.
One study that compared these treatments collected some data on resource use, but these were not valued.
BH carried out the extracorporeal treatments, collected the serum samples, and was involved in revising the manuscript.
YL conducted the in vivo treatments, collected the spontaneous tumor development data and performed the immunohistochemical analysis of the tumors.
Data on adherence to treatment are collected prospectively, during the treatment and post-treatment periods.
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