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Another study has previously attempted to accelerate the aging process by means of a more aggressive treatment (a solution of 75%% ethanol and 25%% water for 2 weeks at 23 °C), but even this was unable to truly replicate the oral conditions [24].
For mock treatment a solution of Tween 20 and methanol at similar concentrations was prepared.
Both SBCG interventions were well tolerated after 12 months of treatment A solution of sodium bicarbonate and calcium gluconate is effective on reducing the symptoms associated with OA.
For ABA treatment, a solution of ABA (100 μM) or same volume of water as a control was sprayed onto leaf surface of the tomato plants.
For EDTA treatment, a solution of EDTA was added to the enzyme to give a final concentration of ≥1000-fold ≥1000-foldss of EDTA to ECAO.
After a single application of 25 μg of 7,12-dimethylbenz[a]anthracene (DMBA) in a volume of 20 μl, and 15 days without treatment, a solution of 5 μg of phorbol-12-myristate-13-acetate phorbol-12-myristate-13-acetate phorbol-12-myristate-13-acetate phorbol-12-myristate-13-acetate phorbol-12-myristate-13-acetate
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The particles were then transferred by an ultrasonic treatment into a solution and further transferred to a microscopic specimen grid.
A passivation treatment with a solution with CrO3 (200 mg L−1 of Cr6+) was also used for reference.
SOD1 aggregates were prepared by treatment with a solution containing 20% (v/v) trifluoroethanol (TFE) [24, 25].
Conversion coatings for magnesium have traditionally been based on immersion treatment in a solution containing hexavalent chromium compounds.
When the cells were 80-90% confluent, they were harvested by treatment with a solution containing 0.25% trypsin, thoroughly washed and resuspended in supplemented growth medium.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com