Sentence examples for treated with restriction from inspiring English sources

The DNA fragment was treated with restriction enzymes and ligated into a NcoI XhoI-treated pET-21d vector.

Total genomic DNA (100 ng) was treated with restriction enzymes that exhibit distinct sensitivity to cytosine methylation (Table 1).

The amplified gene and the pET52b vector (Novagen) were treated with restriction enzymes NcoI and NotI, purified and ligated.

This fragment was treated with restriction endonuclease BamHI and cloned into the BamHI-linearized and dephosphorylated plasmid pGLG61 [ 21], resulting in the recombinant constructs pGLG61_UOX.

Briefly, 1  μg of genomic DNA was treated with restriction endonucleases that digested the DNA close to, but outside, the region of interest.

A construct of the murine AQP5 promoter (2021 bp upstream of the ATG start codon) was produced by the fusion of three overlapping fragments treated with restriction enzymes followed by ligation.

We analyzed restriction fragment length polymorphism of transferred plasmids using agarose gel electrophoresis of plasmid DNA samples treated with the restriction endonuclease EcoRI (Roche Molecular Biochemicals, Mannheim, Germany).

Double-stranded PCR products were treated with the restriction enzyme BglII and were cloned into the SfiI-digested fUSE5 vector.

We recruited PS patients who were treated with water restriction.

His hyponatremia was treated with water restriction and with the infusion of hypertonic saline.

However, many patients are not treated with dietary restriction, but instead with only swallowed corticosteroids.