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The crude solutions were treated with reduction by tris- 2-corboxyethyl -phosphine hydrochloride, alkylation by iodoacetamide, and fragmentation by trypsin at 37°C for 12 h.
During follow-up one patient treated with reduction of immunosuppression to achieve viral clearance later died from sepsis due to pneumocystis pneumonia (see Additional file 1: Table S1).
The 3 lateral malleolar fractures and the remaining medial fractures were treated with reduction and internal fixation (malleolar screw on the medial side; lag screw and neutralization plate on the lateral side).
In this group of patients, conversion of immunosuppression to a low CyA plus mTORi based regimen tended to be associated with a better short-term renal outcome compared to patients treated with reduction of existing immunosuppression.
As Dr. Singer himself states in his Letter to the Editor, our results showing that 43.7%% of the children with a dislocated forearm fracture treated with reduction without internal fixation needed a secondary procedure under general anesthesia, are in concordance with those presented in previous publications.
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In each group, roughly the same proportion (25 50%) of patients who had been treated with closed reduction alone needed a secondary reduction (Table 2).
In both groups dislocation was treated with closed reduction (except one case that required open reduction) and immobilization by means of orthopedic brace for 1 month.
Continued instability was treated with closed reduction and splinting in supination or manual reduction of the DRUJ followed by pinning of the DRUJ in neutral forearm rotation for 4 6 weeks (Fig. 3a, b) [4].
The same portion of patients in each fracture type group was treated with closed reduction or internal fixation after open or closed reduction.
Another 44 patients were treated with closed reduction alone, of which 22 patients (50%) required secondary reduction (P = 0.000).
[2], revising 71 pelvic ring fractures (10 type B, 61 type C) treated with closed reduction and percutaneous screw fixation, obtained 69 satisfactory reductions and 62 good or excellent functional results according to the Majeed scoring system [13].
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