Exact(4)
In the model of human bronchial epithelial cells treated with cigarette smoke extract, an increase of intracellular ROS was observed, and the activation of autophagy had a deleterious effect promoting the death of epithelial cells [ 26].
Related to development, maternal cigarette smoking during pregnancy causes reduced birth weight (Suter et al. 2013), and expression of MEG3 is down-regulated in placentae with intrauterine growth restriction (McMinn et al. 2006), as was observed in human bronchial epithelial cells treated with cigarette smoke condensate.
Some in vitro studies (Bodis et al. 1992; Gocze et al. 1999; Miceli et al. 2005) have found inhibition of progesterone production by granulosa cells or luteal cells that were treated with cigarette smoke extract or the alkaloids found in smoke (e.g., nicotine, cotinine, anabasine).
Epithelial cells from bronchial brushings of normal (n = 8), asthma (n = 8) and COPD (n = 8) subjects were grown in air-liquid interface cultures, and treated with cigarette smoke extract (CSE) and/or Th2 cytokine IL-13, followed by Mp infection and treatment with β2-agonists albuterol and formoterol for up to seven days.
Similar(56)
Exponentially growing cultures of BEAS-2B cells or primary bronchial epithelial cells were treated with medium containing cigarette smoke condensates at 0.5 mg/ml for 1 to 8 days changing to fresh smoke condensate medium every 2 days.
In addition, Mr. Kramer said, Mr. Farren had emphysema, "which he treated with alcohol and cigarettes".
The downregulation of several miRNAs was also observed in the lungs of rats treated with typical components of cigarette smoke, such as the tobacco-specific nitrosamine [ 28].
The gene product for AFF3 from the lightgreen module is a tissue restricted nuclear transcriptional activator, formerly known as lymphoid nuclear protein related to AF4, and it was down-regulated with respect to controls in BEAS-2B cells (immortalized human bronchial epithelial cells) treated with low doses of cigarette smoke condensate [ 26].
Patients treated with CIT are often heavy cigarette smokers.
Seven years after the original arrest, Andrew filed a civil suit stating that he had been beaten, suffocated with a plastic bag, burned (by cigarette and radiator), treated with electric shock, and been the victim of the pattern of a cover-up.
Figure 11 shows A549 cells exposed to particulate phase, vapor phase, and whole smoke from the reference cigarette 2R4F and treated with thapsigargin to assess inhibition of splicing.
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