Sentence examples for treated successively from inspiring English sources
Recurrent attacks over the following days were treated successively with sc sumatriptan and/or oxygen inhalation, and progressively diminished in frequency, and ceased about 7 days later.
A human chondrosarcoma cell line, CS-1, was treated successively with increasing concentrations of the marine chemotherapeutic Ecteinascidin-743 (ET-743), yielding a variant cell line displaying a significant degree of resistance to the cytotoxic action of this drug.
The manifold-valued Brownian motion and random point measures on manifolds are treated successively in the same framework, and stochastic analysis of the Brownian motion on a Riemannian manifold turns out to be closely related to classical stochastic calculus for jump processes.
Membranes were then washed with TBST, treated successively with 5% skim milk for 2 hours, and incubated with anti-CK19 antibody (1∶100, Santa Cruz Biotechnology) overnight at 4°C and HRP-labeled anti-goat antibodies (1∶1000, Jackson ImmunoResearch Laboratories) for an additional hour, with successive TBST washing for three times in each step.
Precipitate was treated successively with dry solvent ether and acetone.
After 7 days of culture, CD56dim and CD56bright NK cells were washed twice with PBS (completed with 2% foetal calf serum [FCS]) and treated successively with FITC-, PE-, biotin-streptavidin-ECD-, APC-Cy7-,-Cy7-, and PE-Cy7-conjugated mAbs on ice for 10 minutes and washed with PBS (completed with 2% FCS).
After filtration, the residue was successively treated with ethyl acetate and n-butanol.
Accordingly, titanium was successively treated with magnesium and silver plasma immersion ion implantation & deposition (Mg Ag PIII&D).
After filtration, the aqueous solution was successively treated with ethyl acetate and n-butanol, and then their extracts were concentrated to dryness [12, 13].
Once more, we ran a full leave-one-out crossvalidation experiment, where we successively treated each of the 497 cattle as a test individual and used the remaining 496 cattle as the training set.
DAPI staining was performed after [21] with the following modifications: after fixation in 4% formaldehyde, the wings were successively treated at 17°C with chloroform for 1 h, heptane for 2 h, and 1× PBS/0.2% Tween 20 for 3 h, prior to the overnight DAPI (1∶10000, Sigma) staining at 17°C.
