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A bigger serving of this, over rice, with more gently treated shrimp on top, would have been meal enough.
heat treated shrimp waste.
acid treated shrimp waste.
The highest crude yield of 33%% was observed for the acid treated shrimp waste sample macerated using acetone.
Acid treated shrimp waste was prepared by dipping the sundried and powdered raw shrimp waste in 4 M HCl.
Heat treated shrimp macerated in methanol has also provided with a reasonable crude percentage yield of 32%%.
Similar(43)
It is made by treating shrimp and other crustacean shells with the alkali sodium hydroxide.
Bisulfite-treated DNA was PCR amplified (Qiagen HotStar Taq Polymerase; Bio-Strategy Ltd, AucklaNZ, NZ) and treated with Shrimp Alkaline Phosphatase (Sequenom, Queensland, Australia), heat inactivated, and a simultaneous in vitro transcription/uracil-cleavage reaction was carried out.
Targeted loci were amplified using Taq polymerase, treated with Shrimp alkaline phosphatase (SAP) to dephosphorylate any unincorporated dNTPs, and an extension reaction was performed using iPLEX extension Gold reagents (Agena Bioscience, San Diego, CA, USA) according to the manufacturer's protocol.
In agreement with this, genomic RNAs from ZEBOV and NiV particles lost their IFN-inducing activities when treated with shrimp alkaline phosphatase (SAP).
Subsequently, PCR products were treated with shrimp alkaline phosphatase (iPLEX™ Gold Kit, SEQUENOM) at 37°C for 20 minutes to remove excess of deoxynucleotide triphosphates, followed by 10 minutes at 85°C to inactivate the enzyme.
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