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To hold the treated sections down while the resin dries, they place small bags of lead shot on tissue sheets over the paint.
Bio-ZnONPs' treated sections on day 14 (G I) and day 28 (J L).
Such singular co-localizations were found in both Rho1D4 and Rho4D2 treated sections (Tables 3,4).
Fluorescent densities on each of the antibody treated sections were measured with the NIH ImageJ program as described previously [19], [67].
Images of sections treated with antibodies preincubated with peptide were taken with the intensity of fluorescence gain equal to or greater than images of antibody treated sections.
One cone remained unlabeled in either immunohistochemical protocol (arrow) whereas the other one (arrowhead) was labeled in the preceding and following JH492 treated sections.
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An example is given in Figure 3E 3H where the background staining of the JH455 treated section (Figure 3G) displayed two unlabeled outer segments.
The treated section of brain showed normal glial cells.
The treated section of the kidney showed normal glomeruli with normal Bowman's capsule.
Control experiments consisted of treating sections with PAG10nm alone.
Treating sections for 10 min in 3% hydrogen peroxide quenched endogenous peroxidase activity.
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