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X-ray photoelectron spectroscopy (XPS) mapping measurements have been carried out to show the distribution of oxygen functional groups across sections of the plasma treated line patterns.
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In addition, the width of the plasma treated lines was found to increase with increasing number of treatment repeats.
Results of breeding from female progeny of tetracycline treated lines.
However, there was considerable variance between the behaviour of the treated lines.
Conversely, the remaining treated lines retained the wide divergence between the HpaII and MspI profiles, and so were indistinguishable from the untreated control lines.
However, it was notable that this distinction had been lost in four of the treated lines sampled, but had been retained in others.
Four treated lines (BraRoAZ_11543e2, BraRoAZ_12447e2, BraRoAZ_10261e3, BraRoAZ_10542e3) from the E2 and E3 generations showed a marked loss in discrimination between enzyme profiles relative to the untreated controls.
Conversely, all four females from the two tetracycline treated lines produced increased egg hatch rates and progenic sex ratios that did not differ significantly from 1 1 (Table 4).
The accumulated sludge (including scum cleaned out by scraper, and the sediment sludge) is pumped into a manhole, which join the SMDD sludge treating line.
The effects of line, and low versus high groups of lines (LvsH) were determined using linear models (line) or linear mixed effects models (LvsH; treating line as a random effect) in R (www.r-project.org).org
In principle, line is a random effect (and thus a true variance component), but given that the control "treatment" (i.e., the N2 line) is unreplicated, we treat line as a fixed effect.
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