However, as we noted previously [10], the antibody used here reacts more strongly to the modified eEF-2 than to the native protein (compare the "W" bands in control vs. toxin treated lanes), so that the relative amounts of the two forms cannot be estimated from images like that in Fig. 1b.
However, it also seems that all of the HU treated lanes are underloaded.
However, it also seems that all of the HU treated lanes are under loaded.
The nature of the higher molecular weight band in the DSS 4 treated lanes is currently not clear.
For SOXL 1 treated lanes, an additional band consistent with addition of at least one copy of SOXL 1 (∼1.6 kDa) to GST monomer (∼27 kDa) is apparent.
Fig. 6 Polymerase chain reaction results of fresh cockle samples (Lane M: 2 μl of 100 bp DNA ladder, Lane 1 3 untreated unspiked samples, Lane 4 6 treated unspiked samples, Lane 7 9 untreated spiked samples, Lane 10 12 treated spiked samples, Lane C+ positive control (V. parahaemolyticus toxR gene), Lane C− negative control (sterile distilled water).
Conditioned medium from untreated (lane 1, Fig. 7) and control siRNA treated cell (lane 2, Fig. 7) contain the same amount of total MMP-2.
After 144 hours of incubation, the telomere length of chondrocytes was significantly shorter in H2O2 treated groups (lane 4 in Fig. 7a,b) than in control group (lane 2 in Fig. 7b).
Cells were then treated with (lanes 4 6) or without (lanes 1 3) BMP7 (10 ng/mL) for 24 h.
When programmed with repRNA template, the activity of the solubilized and partially purified tombusvirus replicase was reduced to 23% by QC treatment (Fig. 2C, lanes 3, 8, 9), whereas CPZ had only minor effect (lanes 2, 4, 5) when compared with the DMSO treated sample (lanes 1, 6, 7).
