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Mice were then either infected intranasally (IN) with 107 CFU of M. pneumoniae or treated IN with the indicated molar amount of rCARDS toxin or HI toxin.
BalbC/J mice were treated IN with 175 or 700 pmol rCARDS toxin or carrier fluid, and then baseline AO and AHR were determined on day 2. As shown in Figures 10A and B, 700 pmol rCARDS toxin induced statistically significant (p<0.05) changes in both AO and AHR two days after toxin exposure.
Mice were treated IN with rF-ptn alone or rF-ptn mixed with NE adjuvant (NE + rF-ptn).
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