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Having established optimum conditions for the formation of the ketene intermediate, we applied the in situ ketene trapping reaction to the acylation of a range of amines (Table 1).
The resultant polymers were grafted onto carbon black surface through a radical trapping reaction at 130 °C in DMF.
The level of 2-MQ, a product from the reaction of MG and o-PDA, represented free MG remaining from the trapping reaction.
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In the case of oxidised polyamine compounds, the corresponding reaction products were determined using analytical methods (coloured trapping reactions, mass spectrometry, IR spectroscopy, thin layer chromatography).
Traditionally carbenes are viewed as so reactive that were only studied indirectly, e.g. by trapping reactions.
Successful HDDA and Diels Alder trapping reactions were observed using CHCl3 as a solvent, which is an excellent hydrogen atom donor, pointing to a lack of radical character in the cyclization.
Trapping reactions of oligonucleotides with PFBHA were performed in water at room temperature for 1 h using 5 μM DNA and 500 μM PFBHA in a 10 μL incubation volume, followed by HPLC/Q-TOF MS analysis, as described previously.
By trapping the reaction intermediate, the reaction mechanism under high pressure CFC-12 at a Pb electrode was presumed to proceed via dimerization of difluorocarbene.
COMT (caffeic acid O-methyl transferase) downregulation in poplar results in the incorporation of 5-hydroxyconiferyl alcohol into lignins via typical radical coupling reactions, but post-coupling quinone methide internal trapping reactions produce novel benzodioxane units in the lignin.
The entire microfluidic assay process, including beads trapping, immunoassay reaction, washing and pumping was completed in a centrifugal platform.
As well as being concentrated by trapping through reaction with formaldehyde 4, cyanide can also be concentrated through complexation to ferrous ions giving ferrocyanide.
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