These insertions included lethal, semi-lethal and sterile as well as viable lines that showed an enhancer-trap pattern.
Additionally, depending on the new chromosomal location of piggyBac, a new insertion might show a novel enhancer-trap pattern.
To confirm the observed TRAP pattern and to determine the sequence of the telomerase product, we sequenced TRAP products of 36 algal strains from the chlamydomonadalean clades Golenkinia, Radicarteria, Oogamochlamydinia, Reinhardtinia, Chloromonadinia, Monadinia, and Moewusinia, and of three species from the sister group Sphaeropleales (supplementary fig. S1, Supplementary Material online).
Due to a low telomerase activity/processivity resulting in an unclear TRAP pattern (table 2 and fig. 5 E – G ) and a failure in cloning of the TRAP products (except TEL157), the question about the steps in telomere evolution of the Stephanosphaeria remains open.
This study determined if different trap patterns which reflect flower shape may affect the attractiveness to thrips.
The shapes of Dendrobium and Mokara orchid flowers were characterized and the numbers of flowers per raceme were counted in inflorescences in order to design realistic trap patterns.
Up to this point, we have considered solar cell architectures with periodic light-trapping patterns.
To detect enhancer-trap patterns in embryos, we dechorionated embryos derived from F3-crosses.
Of these, 421 proved to be recessive lethal, 75 were semi-lethal, and eight indicated recessive sterility, while 505 showed new enhancer-trap patterns.
We have identified many insertion lines that are either homozygous lethal, homozygous sterile and/or show enhancer-trap patterns at various developmental stages.
Although we observed a bias for certain patterns (i.e. certain central nervous system patterns, segmentally-repeated stripes in embryos, or small dots at the hinges of extremities in larvae and pupae), we identified 505 unique enhancer-trap patterns.
