Exact(2)
The relative resistances were modulated by changing the dimensions of the cell trap membrane chamber.
Amphipols offer the ability to trap membrane proteins in detergent-free aqueous solutions, offering new possibilities for the structural analysis of membrane proteins by ESI-MS.
Similar(58)
Thanks to the SLO permeabilization protocol, we could actually visualize the human sperm acrosomal calcium store with Fluo3-AM, something not achievable in non-permeabilized sperm, where cytosolic esterases remove the AM moiety, trapping membrane impermeant Fluo3 in the cytosol.
The ability to directly detect Ti-2 intermediates was subsequently exploited for a new protein engineering technology called MAD-TRAP (membrane-anchored display for Tat-based recognition of associating proteins).
Processing mutations likely trap polytopic membrane proteins like P-gp and CFTR in protease-sensitive conformations with incomplete packing of the TM segments.
This could be important for trapping certain membrane proteins at the tip of the endocytic bud, as well as for preventing the entry of other membrane anchored molecules into this region (see Figure 6), although additional in vitro and in vivo work is required to reveal the exact organization and function of BAR domain proteins at the endocytic sites.
Lobell et al. [ 68] proposed that spherical shapes have a small advantage compared with rod-like shapes with regard to BBB penetration, they attributed this to the membranes that are largely made from rod-shaped molecules and rod-like shape may become more easily trapped within membrane without exiting into the brain compartment.
The presence of LAMP1 in the TRAP + vesicle membrane does not alone allow a direct assignment of the nature of the vesicles.
The extracorporeal circuit, required to infuse a concentrated (40%) lactic acid solution, consisted of a centrifugal pump (Jostra rotaflow, Maquet, Hechingen, Germany), a gas trap, a polysulphone membrane dialyzer (F8HPS, Fresenius, Bad-Homburg, Germany) and /4 to /16 inch inner-diameter tubing.
The base idea behind the method is to use the proteins existing electrostatic charge by developing a polar potential difference across the clay, while trapping the substrate and allowing the protein to deadsorb and become trapped on a membrane.
Majority of AuNP 2-III and AuNP 2-IV are trapped near the membrane and only some enter the cell.
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