Sentence examples for trap buffer from inspiring English sources

TRAP staining was performed using TRAP buffer (50 mM sodium tartrate and 0.12 M sodium acetate, pH 5.2) with naphthol AS-MX phosphate and fast red violet LB salt (Sigma-Aldrich) as described previously [ 23].

Each reaction was performed in 50  μl reaction mixture containing 10 × TRAP buffer, dNTP mix, TS primer, TRAP primer mix and Taq polymerase.

Each 25  μl reaction mixture contained 10 × TRAP buffer, dNTP mix, TS primer, TRAP primer mix, Taq polymerase, and 0.1  μg protein extract.

To obtain semiquantitative levels of telomerase activity, an internal telomerase assay standard (ITAS; 25 attograms), amplified by the same 2 primers used for the telomerase activity assay, was included in the TRAP buffer.

To measure TRAP activity, multinucleated osteoclasts were fixed in 3.7% formalin for 5 min, permeabilized with 0.1% Triton X-100 for 10 min, and treated with TRAP buffer (100 mM sodium citrate, pH 5.0, 50 mM sodium tartrate) containing 3 mM p-nitrophenyl phosphate (Sigma-Aldrich) at 37°C for 5 min.

To extract protein from exfoliated cells, the pellet obtained on centrifugation was resuspended in ice-cold TRAP buffer (tris idrossimetil)amminometano cloridrato (ph 7.5) 10 mM, MgCl2 1 mM, ethylene glycol tetraacetic acid (EGTA) 1 mM, phenyl methylsulfonyl 0.1 mM, β-mercaptoethanol 5 mM, CHAPS 0.5%, and glycerol 10%) and incubated on ice for 1 h.

Culling, trapping, buffer zones and the wrath of the Prince of Wales have so far failed to stop the grey squirrel pushing its smaller red cousin towards extinction in England and Wales.

Using standard glass microfabrication techniques, a microimpinger consisting of a gas sample inlet, gas delivery arms, and an extraction reservoir was used to extract dilute hydrogen cyanide vapor from a gas delivery system using a basic aqueous trapping buffer.

After 3 days of treatment, cells were harvested, washed with PBS and lysed for 30 minutes on ice in trapping buffer (PBS pH 7.4, 0,1% SDS, 0,5% NP40, 0,5% Sodiumdeoxycholate, 1 mM DTT and Roche complete protease inhibitor cocktail).

The lysates were incubated for 2 h at 4°C with either GST or GST-PTP1B-WT or GST-PTP1B-D181A GST-PTP1B-D181A GST-PTP1B-D181A beads, then the beads were washed 4 timutantth trapping bufusion

Passive encapsulation of water-soluble drugs depends on the ability of liposomes to trap aqueous buffer containing a dissolved drug during vesicle formation.