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Some divisions are not oblique to the AV axis; these divisions are either transverse (spindle parallel to the embryo's equatorial plane) or longitudinal (spindle perpendicular to equator).
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Dashes represent the approximate locations of the cross-sections shown in C. (C ) Longitudinal and transverse spindle architecture.
We isolated one new recessive allele of plk-1, or683 ts, which appears to only partially reduce gene function as the most penetrant phenotypes we observed at the restrictive temperature were mis-oriented (transverse) P0 spindles and binucleate cells at the two-cell stage (Figure 8).
10) In Materials and methods ("Quantifying microtubule organisation and the transverse stiffness of spindle"), when the buckling force F is introduced, the equation given corresponds to a beam with constant cross-section along the beam.
The Cross-Sectional Microtubule Organisation Enhances the Spindle's Transverse Stiffness.
This reduction in microtubule numbers, in turn, leads to the decrease in the spindle's transverse stiffness.
The estimates of the spindle's transverse anisotropy ratio reveal that all of the anaphase B spindles have low mechanical anisotropy throughout their extent apart from localised stretches.
The transverse organisation of the spindle is then determined by detecting the positions where microtubules intersect a plane halfway between and perpendicular to the points that define the spindle axis.
Furthermore, the dramatic remodelling of the spindle's transverse architecture at the boundary between the midzone and flanking regions of the spindle further suggests that the cross-linkers that bind to the polar regions also form rigid bridges between pairs of microtubules.
The polymer mass conservation gives rise to a quadratic decay in the spindles' effective transverse stiffness (EIeff ∝ Ls−2, see 'Materials and Methods').
In addition, the NCC fails to rotate causing the P0 spindle to assemble transverse to the anterior-posterior embryonic axis (Fig. 4A).
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