We have set some monitoring points (at certain transversal sections).
In Fig. 6, line profiles (c, d) through the bone marrow uptake observed in Fig. 5a c and the dorsally located tumor in Fig. 5c, d are demonstrated, together with the SARec reconstructed transversal sections through the tumors (a, b).
Frozen tissue was cut in transversal sections of 10 µm at intervals of 30 µm.
Alternatively, thoraces were embedded in OCT and transversal sections (12 µm) were taken with a Leica CM 1510S cryomicrotome.
Images were taken from transversal sections after a single bolus (0.5 ml) of SonoVue contrast agent (Bracco, The Netherlands).
Transversal sections (8 µm thick) were obtained by cryostate at −20°C (LEICA, CM1850UV) and placed onto polysine-coated microscope slides (Menzel Gmbh&Co).
For lignin analysis, transversal sections of pericarps were stained for 2 min in a 2% phloroglucinol solution in 95% ethanol, and then photographed in 37% hydrochloric acid.
Thus, on mammary duct transversal sections, they showed punctate staining at epithelial cell-cell contacts, in the apical part of the lateral membrane.
The nm23-M1−/− mutant nipples appeared histologically normal when observed in transversal sections (not shown), in particular, the histological hallmark of nipple maturation was present and appeared normal.
Coronal, sagittal and transversal sections through the specimen allow retrieval of precise anatomical information down to single cell level (Video S4).
To this end, transversal sections of the UGS from a mouse embryo at day E18.5 were double-immunostained for p63 and FASN.
