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Therefore, a tissue-matched normal sample lacking SB transposition was processed alongside the tumors in order to distinguish actual footprints from endogenous sites with the same sequence.
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Although illegitimate recombination and aberrant transposition are "abnormal" processes, their consequences may have an important impact, since gene duplication and exon shuffling are major processes in gene evolution [ 90].
It is for the first time that mGing active transposition was demonstrated under γ-ray irradiation or in cell re-differentiation process in rice.
Transposition was performed as described previously.
First, the abundance of rearrangement events within the family suggests some mechanism that facilitates gene order mutation; transposition is one such process.
Transposition is an exponential process: if the transposition rate per copy is constant (fig. 1 A), the number of new transpositions increases with the copy number (fig. 1 B).
Marine mammal Brucella species as well as B. ovis and Brucella sp. NVSL 07-0026 share the presence of a high number of IS 711 elements, and it has been demonstrated that IS 711 transposition is still an active process in B. ovis and B. pinnipedialis [ 38].
This process, called transposition, is catalyzed by transposases, coded for by TEs themselves.
As transposition is a "cut-and-paste" process, it is not likely that transposition results in a duplication events unless it is associated with lateral gene transfer (LGT).
Paquin and Williamson reported that Ty transposition is temperature-sensitive and that this process is enhanced at temperatures below the optimal growth temperature [ 11].
However, if transposition is mechanistically linked to replication, the interplay between the two processes could be reflected in detectable chromosome-wide patterns.
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