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The R-dependent increase of the transposition product from hour 12 to hour 24 (Figure 3B) was likely due to selection against the cells without transposition through restriction enzyme action on them (Figure 1B (i)).
The likely process underlying the selection is R-mediated inhibition of growth of the cells without RM transposition through the cleavage of chromosome without sufficient protection of restriction sites due to the absence of methylase as illustrated in Figure 1B (i).
These two genetic elements are necessary for bacterial IS91 (insertion sequence 91) transposition through a rolling circle mechanism.
This chromosomal editing method is based on replicative transposition through the formation of a cointegrate that can be resolved in a recombination-dependent manner.
The mobile component of the genome is represented by sequences, called transposable elements (TEs), which are potentially able to change their chromosomal location (transposition) through different mechanisms.
The CACTA transposons are characterized by terminal inverted repeats (TIRs), target-site duplications of conserved length and transposition through a DNA intermediate.
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Some loci are ubiquitous and, after an ancient origin through transposition, originated through descent from the ancestral trypanosomatid.
However, if pPDL2 is laterally transferred into hosts that do not support its replication, the opd cluster appears to integrate into the host chromosome, either through transposition or through site-specific integration.
This also holds on for TEs, which can be viewed as selfish DNA: the ultimate parasite [ 38], which is able to propagate itself through vertical transmission, through intra-genomic transposition, and through horizontal transfer.
The duplication events occur through transposition events and through various other events leading to segmental, whole-chromosome, or whole-genome duplication (Zhang 2003; Hurles 2004).
Study design: We evaluated the English articles on laparoscopic ovarian transposition identified through a MEDLINE search.
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