Sentence examples for transposition technology from inspiring English sources

In addition, the in vitro transposition technology and the ability to electroporate functional transposition complexes extended the types of organisms that could be analyzed beyond those that are the standard subjects of molecular geneticists.

Finishing steps were performed using primer walking of clones, PCR and in vitro transposition technology with the Template Generation System II Kit (Finnzyme), corresponding to 252, 32 and 8,404 additional reads, respectively.

For the finishing phases, we used primer walking of clones, PCRs and in vitro transposition technology (Template Generation System™ II Kit; Finnzyme, Espoo, Finland), corresponding to 634, 66 and 2,539 additional reads, respectively.

For the finishing phase, we used primer walking on clones, polymerase chain reaction (PCR) and in vitro transposition technology (Template Generation System II Kit; Finnzyme, Espoo, Finland), generating 359, 162 and 533 additional reads, respectively, for Xp_G6 and 701, 823 and 3,701 additional reads, respectively, for Xd.

For the finishing step, we used primer walking of clones, PCR amplifications and in vitro transposition technology [Template Generation System™ II Kit (Fisher Scientific, Illkirsh, France) or Hypermu < Kan-1 > (Tebu-Bio, Le Perray-en-Yvelines, France), corresponding to 814, 33 and 17,975 reads, respectively.

Most laboratories have preference in using either the E. coli transposition-based recombination bacmid technology (e.g. Bac-to-Bac®) or homologous recombination transfection within insect cells (e.g. flashBAC™).

Targeted transposition can be a powerful technology for safe transgene integration in human therapeutic applications.

The highly efficient Mu-based technology of the in vitro DNA transposition has been developed as a convenient tool for the functional analysis of genes, genomes, and proteins because of the accurate nature reaction and the low stringency for the target preference (Haapa et al. 1999; Haapa-Paananen et al. 2002; Savilahti et al. 1995; Savilahti and Mizuuchi 1996; Turakainen et al. 2009).

The transposition reaction was performed according to manufacturer's recommendations (Epicentre Technologies, Madison, WI) with 200 ng of target DNA and a molar equivalent of purified transposon.

During this period, molecular biology had developed significant new technology, and scientists were able to show the molecular basis for transposition.

Development of this technology for a wide range of hosts requires deep understanding of transposition mechanisms of a variety of elements.