Sentence examples for transposition target from inspiring English sources

In the cell, Tn7 uses two distinct transposition target site selection pathways encoded by five genes (TnsABCDE).

The blue boxes are exons 1, 2, and 3 (right to left) of the p1 gene, and the green/black triangles are the transposition target site.

Second generation CAR19 constructs, under the control of the human EF1α promoter and flanked by IR/DR transposition target sequences are being used in trials of autologous and alloegenic trials against B cell malignancies (Singh et al, 2014).

One of the characteristics frequently found for T n5 transposition target sites is the palindromic structure of the insertion site, and also, there is a frequent occurrence of GC pairs at each end of the Direct Repeats [ 33, 34].

In Mu transposition, the target DNA sites are selected with a low stringency of sequence specificity [15], yielding essentially random distribution of integrations along longer regions of DNA [14], [16].

As the initial transposition reaction target DNA, we used a KpnI digest of the identified BAC clone (110 kb), which contained the exon 3 of the mouse Cdh22 gene in a 9 kb fragment.

Thus, such spacers might be suggested to regulate IS transposition by targeting the mRNA of transposases, leading to the regulation of genome rearrangements.

We have accomplished this by targeted transposition, but targeted transpositions typically comprise a few percent of new insertions and require a P-element at the target site.

To discriminate between potential DNA transposition at TTAA target sites and alternative mechanisms of chromosomal integration, we classified genomic insertions based on target sites containing TTAA and those containing other sequence motifs (Table 3).

CLoNe uses a cocktail of labelling, targeting and transposition vectors that enables targeting of specific subpopulations of progenitor types with a combination of fluorophores resulting in multifluorescence that describes multiple clones per specimen.

The locations of the inserted elements varied, and all integrations involved canonical cut-and-paste transposition into TTAA target sites, as expected when using piggyBac transposons (Fraser 2000).