Sentence examples for transposition systems from inspiring English sources

However the EZ-Tn5 system possesses two important advantages, i) a high transposition frequency (e.g., reportedly ∼100-fold higher than phage Mu-based systems) and ii) the highest degree of "randomness" for insertions among commercially-available transposition systems, including phage Mu (www.epibio.com).

These may also lead to improvement of the efficiencies of mariner transposition systems as genome manipulation tools.

Retroviral integration shares a common set of intermediates with many DNA transposition systems (Li et al, 2006; Li and Craigie, 2009).

All transposon-mediated mutagenesis approaches employ efficient transpositional recombination machinery, which catalyzes the joining of the transposon DNA into target DNA, and the most useful transposition systems integrate into a nearly random selection of target sites [ 24].

Obstacles to transformation of obligate intracellular pathogens include: DNA delivery while retaining viability of extracellular bacteria, efficient reintroduction of the transformed bacterial population into host cells, selection (given the limited number of antibiotics ethically applicable to a pathogen), and the limited efficiency of homologous recombination and transposition systems.

The development of robust and efficient in vivo transposition systems [ 2, 61- 63], as shown here, or the use of lentiviral transgenesis, as recently described elsewhere [ 64], open new exciting possibilities to conduct such screens in an efficient and affordable manner.

The stable transfection rate is inversely related to the size of circular donor, suggesting that a MC-based SB transposition system benefits primarily from an increased cellular uptake and/or enhanced expression which can be observed with DNA MCs.

As a control in vitro transposition system, we chose the EZ::TN system to insert Tn5 into our target plasmids.

The plasmid containing pcrV with a type III promoter for exoS, pUCP-pS-pcrV (ampr), was mutagenized using an in vitro transposition system, which results in the insertion of a kanamycin marker (kanr) flanked by NotI sites.

The GPS-1 in vitro transposition system (New England Biolabs) was used as directed by the manufacturer to insert transposon Tn7 into plasmids containing 9 or 108 GAATTC repeats.

Original strains and plasmids for the phage-based transposition system were obtained from Merck Research Laboratories [ 35].