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The transposition rate was measured as described in the prior study18.
The transposition rate was determined by dividing the galactose colony count by the total number of cells plated.
It also retains the insertion activity in mammalian cells with a plasmid to chromosome transposition rate about 10-fold higher than random integration.
(iv) Mutations that influence DNA and protein-protein interactions generally reduce transposition rate, although most hyperactive mutants are also located on the protein surface, including residues with protein-protein interactions.
Nevertheless, unless Galileo has an unusually high transposition rate, this explanation is insufficient because Galileo is not the only TE family transpositionally active in the D. buzzatii genome (at least another eight TE families must be active; Table 3).
Although statistically significant, diversity-dependence remains moderate, and affects the transposition rate only marginally (the current transposition rate for all families is more than 85% of the estimated initial transposition rate when one copy only was present in the genome).
We call this elevation of the transposition rate a transposition burst.
The present transposition rate thus appears to be very low in C. orientalis.
This corresponded to a 7% apparent germline transposition rate (1st screen in Table 1).
As a result, a constant transposition rate mainly generates recent copies.
These assays involve both conjugation and transposition and are therefore likely to underestimate the transposition rate.
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