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This pattern is best explained by a high turnover of insertions resulting from a balance between the transposition of new elements and the selective loss of deleterious alleles.
If autonomous copies are more deleterious to the host than nonautonomous ones, it is plausible that they fail to reach fixation and that their very young age reflects a high rate of turnover where the transposition of new copies is countered by the selective loss of deleterious copies.
However, the few analyses we have on the occurrence and nature of the intervening sequences have demonstrated the presence of many repetitive elements, or rests of transposable elements in these regions, suggesting that one mechanism controlling the cluster size might be through the expansion of repetitive sequences or the inclusion (transposition) of new mobile elements [ 39].
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Next, you mentioned the transposition of the new Eurojust decision.
In the future, it would be good to compare the efficiency of transposition of the new element with sleeping beauty or some previously isolated Tc1-like element, although these Tc1-like elements such as SB and FP were seldom used for fish transgenesis.
Given the high efficiency of transposition, the number of new insertions identified in on-going remobilisation efforts (~ 10 per week) exceeds our current capacity to keep, expand and cryopreserve all of them.
In three of the 28 cases studied, the active BES was replaced by duplicative transposition of the entire new BES.
The duplication and/or transposition of retroelements into new sites may directly affect gene structure, and the presence of multiple copies of these elements throughout the genome could have long-term effects on recombination events and a more subtle influence on gene expression (Feschotte, 2008).
Transposition is an exponential process: if the transposition rate per copy is constant (fig. 1 A), the number of new transpositions increases with the copy number (fig. 1 B).
Evaluation of new transposition methods should be considered if their eventual products require strictly unbiased representation of input DNA.
In this case, we cannot discard the existence of new transposition events in founders induced by the new environmental conditions encountered as previously proposed by other authors [ 10, 11].
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