Understanding if the HeT-A copies capable of transposition were located in a specific region of the telomere was until now a puzzling question.
For example, P-element has subterminal transposition enhancers located outside the short TIR, whereas the secondary binding sites of Sleeping Beauty and Bari-like elements exist as tandem repeats within longer, bipartite TIRs (Ivics et al., 1997; Moschetti et al., 2008; Rio, 2002).
In such a dynamics, the median transposition event is roughly located at mid-course of the amplification time-span.
Genome-wide analysis showed that exposure to 0.5 mg/L imipenem mediated the transposition of IS Aba1, located upstream of the blaOXA-95 gene, resulting in the overexpression of the blaOXA-95 gene.
The MAT locus in yeasts was considered a "deletion hotspot" with a continued process of evolutionary deletions, gene truncation and transpositions on chromosomal genes located beside the MAT locus after the mating-type switching event (Gordon et al. 2011).
In addition, our study is one of the few that have identified blaESBL genes in the chromosome, which might respond either to plasmid integration or transposition driven by IS Ecp1 located upstream from the blaCTX-M-15 gene (25, 39, 40 ).
DNA sequence analysis of the 111 kb IncI1 EHEC virulence plasmid from the human-derived enterohemorrhagic E. coli strain, 6877, by Venturini et al. found the dfrA5-IS26 confinurathis isolate isolate was within a Tn21-derivative [22], which confirms PCR results indicating the integron-IS26 configuration is located adjacent to Tn21 transposition genes in this strain (Table 2).
The transposition event at position 992097 is located 45 base pairs upstream of the stop codon of an ankyrin repeat protein gene (APH_0928).
It seems very unlikely that this Ac was inserted through a simple transposition event, because the insertion site is located precisely at the duplication junction that is generated by RET, and an independent Ac transposition would not be expected to insert into precisely the same site.
The Tn21 transposition genes tnpM, tnpR and tnpA were located downstream of the intI1 gene.
