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Thus, most high coverage transposon libraries in S. aureus typically utilize a temperature sensitive plasmid containing the transposon and require high-temperature plasmid-curing steps to remove the plasmid delivery vehicle after transposition has occurred [ 29- 34].
For the PCR-based analyses, the more recent activity of npiggy1_Mm made it the most suitable marker for testing whether transposition has occurred in the Microcebus genome before or after the hypothesized divergences with L. catta and C. medius.
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From this comparison (and confirmed by others not shown), we can see that one of these tRNAs, trnD, remains in the ancestral condition in these two cephalopods, with the transposition having occurred in the polyplacophoran, whereas all other changes are derived for the cephalopods from that order parsimoniously inferred to be basal for the Mollusca.
Although the latter mode is currently not well explained by a molecular model, we have assumed that single tRNA transpositions have occurred in all cases in which tandem duplication and random deletion seems to be an implausible explanation, e.g. when only one tRNA gene found a new position distant from its original position, or on the other strand.
Thus, we cannot formally conclude on whether transposition of IS607-like sequences has occurred (and is still occurring) in any of the eukaryote genomes considered in this study.
Our results clearly show that the transposition of a sex-determining gene, sdY, has occurred in Atlantic salmon.
gambiae Ty3/gypsy proviral insertions are ≥99% identical with the consensus, suggesting that transposition events have occurred recently in about three-quarters of the Ty3/gypsy families [15].
The authors suggest that specific inversions, rDNA amplification, and locus transposition may have occurred during the process of Oryza evolution.
The time-span of each lineage is reported on Fig. 5a, with an indication of the time at which half of the transposition events have occurred.
Possible elevated rates of gene duplication and transposition could have occurred along the gymnosperm lineage to cause this genome expansion, with evidence to date suggesting that these events were ancient [ 77].
Additionally a transposition event having occurred in one of the barley tillers during the development of the F1 plant may lead to several but not all F2 siblings showing the same new GT DsB insertion (for example see Figure 2a, GT54/3 5).
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