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Other options could be based on tissue transposition directly to regeneration-desired sites without stem cell extraction and long-term culture.
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Transposition efficiency is directly dependent upon increased transposase levels up to a certain threshold level [37]; therefore, our results may reflect this increased transposition efficiency and a corresponding deleterious effect on viability due to increased integration events with higher probability of disrupting essential genes and/or regulatory elements.
There is a demonstrated decrease in transposition efficiency that is directly proportional to transposon size and SB transposase expression levels over a certain threshold (overproduction inhibition) [27], [37].
Mutant colonies were initially isolated directly from transposition plates (post-conjugation) and three times to ensure purity were sub-cultured under antibiotic selection on blood agar plates anaerobically.
Base flipping in Tn5 transposition could not be detected directly using the permanganate assay because the base was protected in a hydrophobic binding-pocket in the protein.
Conversely, the piRNA machinery will act by directly limiting the transposition rate, and thus the rate of production of new insertion alleles.
The aliasing removal procedure has been directly inserted into the transposition algorithm.
Transposable elements (TEs) are known to induce chromosomal rearrangements indirectly through ectopic recombination or aberrant gene conversion and directly by an alternative transposition process [7].
Consequently, the extent to which an increased Ac dosage results in nonspotted kernels may be Ac element specific but not directly related to Ac transposition frequency.
Such activation can potentially directly disrupt genes through transposition into their vicinity 22, 23 or because transcription from LINE-1 promoters can affect neighboring genes 24, 25.
The duplication and/or transposition of retroelements into new sites may directly affect gene structure, and the presence of multiple copies of these elements throughout the genome could have long-term effects on recombination events and a more subtle influence on gene expression (Feschotte, 2008).
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