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One of the advantages of the use of 12-dimensional chroma vectors in the chromagrams is that the transposition amount of modulation can be naturally represented only by the amount ζ by which its 12 elements are shifted (rotated).
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The scanner almost never reads the numbers wrong, he added, whereas "manually, you are prone to transposition or entering the wrong amount".
PiggyBac transposition activity increases as the amount of piggyBac transposases increase until reaching its peak in cells transfected with 200 ng of helper plasmids.
We have observed that (1) the Tol2 transposase (but not piggyBac) is highly sensitive to molecular engineering; (2) the piggyBac donor with only the 40 bp 3'-and 67 bp 5'-terminal repeat domain is sufficient for effective transposition; and (3) a small amount of piggyBac transposases results in robust transposition suggesting the piggyBac transpospase is highly active.
All three sets of transposition reactions contained the same amounts of transposase and supercoiled plasmid substrate.
We have designed a system to silence expression from nontransposed sequences, making it possible to determine more specifically the amount of expression resulting from transposition.
The transposition rates of these TEs depend on the amount and type of the TEs present in the genome; they are not constant over time, but are subject to amplification bursts in certain species and populations [ 2].
When we increased the amount of transposase by 10-fold, the transposition efficiency of mPB improved 2.4-fold.
However, increasing SB100 transposase expression resulted in reduced transposition efficiency compared with that obtained at the lower amount (1.2-fold decrease).
However, for this particular transposase, we also observed an inhibitory effect of increasing the amount of transpose-expressing plasmid, indicating that transposition catalyzed by this transposase in cultured keratinocytes is regulated by overproduction inhibition.
The IE1 transactivator (hr3IE1) was included during transfection at a tenth of the total DNA amount to determine its potential to improve promoter activity or transposition frequency [ 29, 30].
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