The MB6009 Minos insertion in the CLN3 locus (39) was excised with the Minos transpose using a standard crossing scheme.
It has been reported that LINE elements with a non-functional mutation of its reverse transcriptase gene are still able to transpose using the proteins coded by a functional LINE element [ 29, 30].
Contrary to the majority of TEs that transpose using transposases whose active site is composed of a triad of acidic residues (DDE transposases), known members of the IS 200/IS 605 family lack terminal inverted repeats and do not generate larger target site duplications upon transposition [ 3].
Therefore, it is likely that these elements are still able to transpose using the required replication machinery in trans.
Although a more detailed classification system is now being used, two major classes of transposable elements (TE's) exist: retrotransposons, which transpose by using a RNA intermediate, and DNA transposons, which transpose by cutting their genomic sequence and inserting it elsewhere in the genome.
Class II TEs (also known as DNA TEs) have terminal inverted repeats (TIRs) and transpose via DNA intermediates using a "cut-and-paste" mechanism.
Transposable elements (TEs) are divided into two main classes depending on their mode of transposition [ 1]: class I elements transpose through an RNA intermediate using a reverse transcriptase, while class II elements transpose through a DNA intermediate.
With few exceptions, classic "cut-and-paste" DNA transposons have terminal inverted repeats (TIRs) at both ends and transpose using the so-called "cut-and-paste" mechanism (for a review, see [5]).
Unlike the retroelements, DNA-only transposons do not rely on a RNA intermediate, but transpose directly from DNA using a multi-step cut and paste mechanism catalyzed by a transposase that recognizes the transposon DNA by its Terminal Inverted Repeats (TIRs) (Reviewed in [ 3]).
The chimes of a large clock tower were simulated using a wrench, then transposed to a lower pitch.
