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The expression level and transporting activity of transporters may be altered as part of the systemic response (i.e., immune response) to exogenous or endogenous stimuli.
For proton co-transporters, like those expressed in small intestinal epithelium, optimal transporting activity is usually observed at acidic pH condition which is close to physiological conditions in the intestine.
Transporter-mediated therapeutics is achieved via stimulation or inhibition of the substrate transporting activity or potential cellular signaling involved.
We showed previously that the commonly used volatile anesthetic isoflurane increases the transporting activity of EAAT3, the major neuronal EAAT.
Although the uptake activities were tested in yeast inactivated endogenous nucleoside transporter, FUI1, it has certain limitations to examine the role of mammalian plasma membrane transporter because of different membrane structure, intracellular kinases, and posttranslational modification such as glycosylation which could alter the transporting activity of ENT1.
DOI: http://dx.doi.org/10.7554/eLife.03191.017 We further isolated ER/Golgi from Drosophila larvae and tested their iron transporting activity.
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Consequently, mice inactivated systemically for Slc30a8 display profound changes in insulin crystal formation and secretory granule morphology [ 14, 15], consistent with the lower zinc-transporting activity of the transporter isoform encoded by the risk allele [ 15].
Nevertheless, these systems present a so far untapped source of transporters with potential xylose transport activity.
Facilitative glucose transporters (GLUTs) are essential for the glucose transport activity in cells.
(B) Transport activity of hENT1 variants.
First, we investigated the pH dependence of hENT1 transport activity.
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