Finally, while exogenous leucine and insulin were sufficient to stimulate anabolic signalling in cultured myotubes, incubation did not alter gene expression of the amino acid transporters measured.
Its estimated Km of 35.2 μM lies within the K m range of other plant high affinity phosphate transporters, measured by a similar method – the range being from 13 μM to 97 μM [ 13, 14, 21].
In order to assess the expression profiles of transporters measured in whole intestinal tissue with microarrays, we verified the mRNA levels of nine transporters (Abca1, Abcc1, Abcc3, Abcg8, Slc10a2, Slc28a2, Slc2a1, Slc34a2 and Slc5a8) measured in the same RNA sample preparation by RT-PCR.
In order to determine vardenafil's reversal mechanism for the ABCB1 transporter, we measured the accumulation of the [3H]-paclitaxel in the absence and presence of vardenafil.
Transporter assays measure the flux across various cell lines overexpressing P-gp (eg, Caco-2 and MDRK), and the relative flux (i.e., >2) can be used to define whether a drug is a P-gp substrate.
Using a calibrating set of 30 drugs, the ATPase activity of the two transporters was measured as a function of the concentration of the drugs.
Finally, the mRNA expression of some selected transporters was measured along the crypt-villus axis using laser capture microdissection.
Gene expression of 50 ABC transporters was measured using the Taqman Human ABC Transporter Array Appliedd Biosystems, Invitrogen Life Technologies) according to manufacturer's instructions.
All nine members of the LIV-1 subfamily of ZIP transporters were measured in our model of tamoxifen-resistant breast cancer using Affymetrix arrays.
(B ) The functionality of the transporters was measured as growth of the yeast Δ mep1,2,3 mutant transformed with AMT1 3-FP fusions AMT1 3-FP on solid media containing 2 mM NH4Cl or 1 mM arginine (growth control) as the sole nitrogen source fusionsee dand.
