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They remained in use until 1985, when passenger transport was terminated.
In the early 1950s, discussion started about closing the line, and on 1 November 1955, all passenger transport was terminated, after a decision in parliament on 26 May 1955.
Amino acid transport was terminated by rapidly washing the cells twice with ice-cold PBS.
After 5 minutes, transport was terminated by washing the cells with ice-cold KRH glucose (KRH glucose containing 25 mM d--Glucose).
Pi transport was terminated by the addition of 1 mL of ice-cold 25 mM Tris−succinate buffer, pH 6.5, and rapid filtration under vacuum (7).
Transport was terminated by immersion in ice-cold KRB containing 80 μM cytochalasin B. Muscles were frozen in liquid nitrogen and processed as previously described (Sakamoto et al., 2005).
Similar(52)
At the end of the cell-cycle, vacuole transport is terminated by ubiquitylation of Vac17, which is then degraded by the 26S proteasome (Yau et al., 2014).
From 1 March 1989, all transport on the line was terminated.
Their employment was terminated.
The contract was terminated.
Magness' contract was terminated.
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