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At the end of the cell-cycle, vacuole transport is terminated by ubiquitylation of Vac17, which is then degraded by the 26S proteasome (Yau et al., 2014).
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They remained in use until 1985, when passenger transport was terminated.
In the early 1950s, discussion started about closing the line, and on 1 November 1955, all passenger transport was terminated, after a decision in parliament on 26 May 1955.
Amino acid transport was terminated by rapidly washing the cells twice with ice-cold PBS.
After 5 minutes, transport was terminated by washing the cells with ice-cold KRH glucose (KRH glucose containing 25 mM d--Glucose).
Pi transport was terminated by the addition of 1 mL of ice-cold 25 mM Tris−succinate buffer, pH 6.5, and rapid filtration under vacuum (7).
After 10 min transport was terminated by addition of 50 μM cytochalasin B. Adipocytes were recovered by centrifugation and washed twice with incubation medium containing cytochalasin B and then solubilized in 0.1 M NaOH prior to scintillation counting.
After disengaging the vacuum, OxlT function was monitored by overlaying trapped proteoliposomes with 0.2 mL of 0.1 mM [C]oxalate prepared in the assay buffer; [C]oxalate transport was terminated after 1 min by vacuum filtration, immediately followed by two 5 mL washes with assay buffer.
Our engagement is terminated!
The pregnancy is terminated by the mother.
Then the iteration is terminated.
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