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To improve the annotation of transporters prior to the key word searches, the two Salinispora genomes were submitted to transportDB [ 41], which annotates transporters according to the transport classification system [ 42].
These genes were subsequently classified into 62 families according to the transporter classification system used in Transport Classification Database (http://www.tcdb.org/).
Furthermore, BLAST analysis of both the pneumococcal and H. influenzae genomes against the transport classification database (http://www.tcdb.org/) failed to identify any other calcium transporters, suggesting that sequences and motifs differ from those characterized so far.
When appropriate, other predictive tools such as InterProScan [17], PFP [18], PSORTb [19], ExPASy ENZYME [20], Helix-Turn-Helix Predictor [21], MEROPs [22], and the Transport Classification Database [23] were used.
To link annotated genes to proteins and proteins to reactions, biological databases such as KEGG, GeneDB, UniProt, BRENDA, Transport Classification Database (TCDB), and TransportDB were used [ 83- 88].
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The basic units of Transporter Classification (TC) are complete transport systems that often include nonhomologous domains and/or proteins.
Identification and classification of transporter proteins in the V. macrocarpon predicted proteins data set was done using 6,099 membrane transport protein sequences downloaded from The Transporter Classification Database (TCDB) [ 105].
Families of transport proteins were compared in detail using the Transporter Classification (TC) system.
Encoded transport proteins were identified by BLAST search against the Transporter Classification Database [ 117] and KO classification [ 118].
Transport information was obtained by cross-referencing BLATSp searches and the Transporter Classification Database (TCDB) [ 45].
Transport protein coding genes were annotated using similarity searches against the Transporter Classification Database (TCDB) [ 91].
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