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Facilitative glucose transporters (GLUTs) are essential for the glucose transport activity in cells.
(B) Transport activity of hENT1 variants.
First, we investigated the pH dependence of hENT1 transport activity.
Thirdly, we studied the proton wires associated with transport activity.
Glucose transport activity was examined in isolated muscles.
The black boxes represent the transport activity of nucleoside-derived drugs for hENT1, whereas the orange boxes represent the transport activity for nucleobase-derived drugs.
The black boxes represent the transport activity of nucleoside and deoxynucleoside for hENT1, whereas the green boxes represent the transport activity for nucleobase.
The transport activity for each substrate was determined within a linear range (5 min).
Furthermore, LY294002 has been found to antagonize transport activity of P-glycoprotein without influencing its expression.
(B) Transport activity of hENT1 for nucleoside-derived and nucleobase-derived drugs.
Disrupting the switching process (e.g., L944N) abolishes transport activity (Fig. 2D).
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