A mixture of 10 g/L α-cellulose in 0.1 M sodium acetate buffer (pH 4.8) was shaken in a transparent 2 mL Eppendorf tube with an inner tube diameter D t = 1 cm on an orbital shaking platform.
On May 16 , 2012 Cooley LLP, Spiegel and Murphy's representatives at the time, sent Brown a letter, calling his actions "a transparent and desperate attempt to shake down Mr. Spiegel and Mr. Murphy for a share in a company to which you contributed nothing".
His transparent threat is like that of a gangster shaking down a store owner, pretending not to know who is responsible for the vandalism that happens to afflict businesses that don't make their payments to the mob.
The erythrocyte precipitated at the bottom of other six tubes could be dispersed after shaking, and the supernatant was achromatic and transparent in the period of 1 h observation.
"New York state government needs to be more open and transparent," Mr. Russo said, "and it needs reformers who will shake it up so it serves more than just the people who have connections".
The changes emerging from the regional shake-up "will enable the investment community to be more transparent, accountable and held to better standards of corporate governance," he said.
After that, he wants to shake up the market by forcing the companies to be more transparent and separating their retail and generation businesses, sparking fury from the major players.
Shake off the nerves with a swim in the freezing cold, eerily transparent waters of the lake or by spotting the hordes of seals that gather on the island.
'Transparent' vs. 'Modern Family' and what Sunday's Emmys say about TV's cultural shake-ups.
The microplate was covered with a transparent lid to counteract evaporation and incubated in the fluorometer (BMG FLUOstar) with continuous shaking (200 rpm, linear mode) between each cycle of repetitive measurements.
