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In contrast, 454 sequences from a chronic HIV-1 infection case (WEAU's transmitting partner, RIER), showed extensive diversity throughout both the epitope and V3 regions (Figs. 3, 4).
The primary trial endpoint was defined as incident HIV-1 infection in a previously HIV-1 uninfected partner ('seroconverting partner') confirmed to be genetically linked to his/her putative transmitting partner ('HIV-1 infected partner') by viral sequence analysis.
We obtained thousands of sequences from 3 early time-points for each of the subjects CH40, SUMA, and WEAU, and chronic-infection sequences from WEAU's transmitting partner RIER (Table 1).
Thus, the data indicating that Ro is lowest in HIV+/HIV− partner pairs in which the transmitting partner has a low-risk GRG status suggest that vaccines directed towards reducing infectivity of the HIV+ host may play an important role in halting epidemic spread.
Of note, there is extreme variation in the viral sequences from WEAU's transmitting partner RIER, which were sampled during chronic infection near the time of transmission to WEAU (Fig. 4A, grey branches); nevertheless, the variants found in WEAU, even at later time points, appear to have evolved from a single founder (Fig. 4A), a further indication that multiple variants were not transmitted.
Recent data has revealed the role of minority viral variants from the transmitting partner in individuals acquiring HIV-1 through heterosexual sex [22], [38], [39], which was our rationale for using deep sequencing techniques in couples whose viruses were initially found to be unlinked.
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The maximum plasma HIV-1 RNA level measured at any point during study follow-up for these three HIV-1 transmitting partners were 3.19, 3.95 and 4.71 log10 copies/mL.
It is likely that we will need to focus on several factors to reduce new HIV infections, including: reducing the burden of undiagnosed HIV infection, educating patients and clinicians to recognize the symptoms of primary HIV, and starting ART in those who wish to in order to reduce the risk of them transmitting to partners.
Characteristics of the transmitting and seroconverting partner affect HIV-1 sexual transmission risk, making studies of HIV-1 serodiscordant couples (in which one partner is HIV-1 infected and the other is uninfected) of significant scientific value [1].
The first term is because of the Alamouti scheme[16], where the transmitting and the partner user transmit using Alamouti scheme, and the second term shows here because of the relay node forwarding network coded bits to the destination[17].
Here, the phasing procedure may incorrectly infer that an allele transmitted by the recipient was instead transmitted by his/her partner; thus the potential NCO allele is not necessarily observed in the grandchildren.
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