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In the Transmit program, genotypes of unaffected siblings (or siblings whose disease status is unknown) are used to infer parental genotypes, thus increasing the power to detect association.
Analysis of haplotypes by the TRANSMIT program also showed that the GGC haplotype was associated with early-onset CHD (p = 7.9 × 10-7).
It is more conservative and has lesser type 1 error when compared with the TDT test implemented in the Transmit program.
The TRANSMIT program provided the observed and the expected number of transmissions for each of the common alleles of the five markers from a parent to an affected offspring.
When one parent is missing, the Transmit program assigns and weights possible haplotypes to the missing parent using the information from the known parent and the siblings of the proband, and averages the probability of all transmissions to the proband.
There was no information from unaffected siblings of the probands for these UK families, which is necessary for the Transmit program to narrow down the range of the possible parental genotypes.
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