In contrast, in MDA-MB-435 and MDA-MB-468 cells, which had relative lower transmigration rates compared to MDA-MB-231 cells, binding of the E-selectin/Fc recombinant was mainly found at sites of cell-cell contacts.
Accordingly, an in vitro blood brain barrier model constructed from heparanase-overexpressing cerebral vascular cells showed impaired transmigration of monocytes compared to a corresponding assembly of control cells.
As a further control, we have tested the migration of A375 (a commercially available melanoma cell line) in our model and we found similar transmigration ability if compared to what was previously reported by other authors for the same cell line in a similar in vitro model (data not shown) [ 53].
First, we found that microinjection of MIP-1α, PAF, or E. coli induced a significant increase in leukocyte adhesion, transmigration, and motility of transmigrated leukocytes as compared to microinjection of saline.
Intravascular administration of plasmin did not alter rolling, firm adherence, and transmigration of leukocytes as compared to controls (at all concentrations investigated; Fig. 6A, C, E).
In response to MCP-1, AGEs treated THP-1 cells also showed an increased transmigration across TNFα activated HMEC-1 monolayers compared to THP-1 cells treated with BSA (Fig. 1E).
While there was no difference in the transmigratory capacity of naïve and memory B cells between the groups, we observed a profoundly increased transmigration of regulatory B cells derived from FTY compared to HC and MS (Fig. 3D; regulatory B cells: HC 3.9%, MS 5.3%, FTY 12.3%).
However, the transmigration was around threefold higher for CD27negative B-cells compared to a twofold increase for CD27positive B-cells.
Indeed, for the TCRζdim subset transmigration in response to CXCL-12 was reduced when compared to cells migrating in the absence of exogenous chemokine.
Adams has modestly described the piece as a "memory space," and compared to many of his previous works the language of "Transmigration" at first seems cool.
