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We report an integrated platelet translocation analysis system that measures complex dynamic platelet protein surface interactions in microliter volumes of unmodified anticoagulated whole blood under controlled fluid shear conditions.
This was initially suggested during the identification of BCL-2 by chromosomal translocation analysis that also revealed the abnormal chromosomal translocation of the MYC oncogene in an acute B-cell leukemia patient [56].
STIM1 translocation analysis was done as described by Luik et al (2008 [27].
For target translocation analysis, cells stained with Hoechst dye were identified as objects in channel 1 and an adjustable mask called Circ was created around every nucleus.
It appears that multisubunit RNAPs present a more challenging target for translocation analysis than T7 RNAP.
Related to this, it is possible that next-generation sequencing might become applicable to translocation analysis in the future.
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Similarly in a patient with an unbalanced X; A translocation, expression analysis of individual loci demonstrated discontinuous silencing of autosomal genes in cis with the inactive X [ 13].
To investigate whether SWT can increase the activation of Nrf2 by inducing its nuclear translocation, immunocytochemistry analysis was conducted for MCF-10A cells treated by the SWT.
All patients were analysed for presence of EGFR and KRAS mutation and for ALK translocation: this analysis included 56 (44.8%) EGFR mutated, 29 (23.2%) KRAS mutated, 10 (8.0%) ALK translocated and 30 (24.0%) EGFR/KRAS/ALK wild type, defined as triple negative.
Cells were analysed using MetaXpress Translocation-Enhanced analysis module.
The diagnosis is confirmed by cytologic examination of ocular fluid specimens and more recently by molecular-immunoglobulin heavy chain (IGH) translocation or cytokine analysis.
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