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These data suggest a quantitative difference in 'readout' as compared with the recombination assays, perhaps because hexamers with mutated subunits are less able to displace a triplex when they have translocated up to it, as compared with being able to activate recombination after translocation up to the XerCD- dif complex.
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Heme-copper oxygen reductases (HCOs) are the last enzymatic complexes of most aerobic respiratory chains, reducing dioxygen to water and translocating up to four protons across the inner mitochondrial membrane (eukaryotes) or cytoplasmatic membrane (prokaryotes).
In considering these points together with the fact that attaching and effacing pathogens employ one T3SS to translocate up to 49 effectors in order to parasitise the gastrointestinal tract [32], it seems possible that Salmonella, using two T3SSs to parasitise the gastrointestinal tract and systemic sites, will encode numerous effectors in addition to the 28 described thus far.
The algal endosymbionts can translocate up to 95% of their photosynthetic products to the hosts, where these compounds are primarily used to meet host respiratory demand [ 7, 8].
This led us to wonder whether the low activity in the triplex-displacement assays results from a failure to displace the triplex efficiently rather than an impaired ability to translocate up to the triplex.
In contrast, the weaker streptavidin was displaced to comparable levels (⩾82%) by all three proteins, showing that they have comparable abilities to translocate up to, and displace, a weak roadblock.
Many Icm/Dot substrates were up-regulated at the post-exponential phase in vitro [10], [54] [56], but translocating proteins up-regulated at the exponential phase should exist because of their diverse functions throughout the infection process.
As a result the fusion protein will be fully translocated and end up inside the secretory pathway.
While conformation 1 is identical to one of the crystal structures, the shoulder in conformation 2 is translocated longitudinally up to 10 Å, resulting in an outward-projected cap.
They showed that different amounts of Fe3O4 nanoparticles were taken up and translocated throughout the root, stem and leaves and indicated the nanoparticle transport pathways and bioaccumulation into the plant system.
For this experiment, we collected juveniles and adults from a common site, tagged them with elastomer, and either returned them to their home site or else translocated them different distances (up to 800 m) away from their home site.
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