During this period, 50 territorial males were captured and translocated at equal numbers (10 per translocation distance) to five distances (50, 100, 200, 400, and 800 m) in all cardinal directions (north, east, south, and west).
The relatively low steady state level of ATP hydrolysis by the trimers indicates that most molecules are not translocating at any given time, presumably because they are not loaded onto DNA, have collided with other translocases, or are simply not active.
Fourth, some substrates may be translocated at efficiencies beyond the detection sensitivity of the β-lactamase assay.
On the other hand, the poorly expressed TEM-Lpg2555 TEM-Lpg28742874 were translocated at high efficiency (Fig. 3).
Further, our results have revealed Lpg2844, a protein with a carboxyl amino acid composition highly different from most substrates can be translocated at high efficiencies (Fig. 4).
Rane and colleagues generated Tg mice expressing a variant of hamster PrP carrying the interferon-γ signal peptide (Ifn-PrP), which was constitutively translocated at low levels [19].
The first explanation is that the gene has been translocated at least twice among F. v. ssp.
With channels formed from WT PA63, all of the LFN variants translocated at essentially the same rate and efficiency as WT LFN, except for the LFN R263C−biotin−streptavidin complex, a negative control.
A VHF radiocollar (Telonics Inc., Lotek Wireless Inc., Advanced Telemetry Systems, Inc., and Telemetry Solutions) with mortality sensor was placed on animals to be translocated at the time of capture to facilitate monitoring after their release in the SAM.
