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Strikingly, addition of ISRIB to stressed cells with pre-formed SGs lead to their quick dissolution (less than 5 min), liberating mRNAs back into the translational pool.
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We used three different data inputs: i) data derived from total RNA, ii) data derived from the translationally active (i.e. polyribosome-associated) RNA pool and iii) translational data that had been corrected for total RNA abundance by taking the ratio of (transcript abundance in the actively translated pool)/ total transcript abundance) cell line for cell line.
We used MANOVAs to determine the differences between the rate of regular and translational head movements pooling all species and within each species.
This result is consistent with the idea that maturation initiates a period of transcriptional quiescence during which events are primarily driven by translational activation of a pool of stored maternal RNAs.
The efforts of universities and their partner teaching hospitals to increase the pool of translational investigators can, thus, be assessed by measuring the number of such awards.
Bridging research and clinical practice by increasing the pool of translational investigators is high on the agenda on both sides of the Atlantic [ 70- 72].
These studies have strongly supported the existence of temporal and spatial concomitant mRNA codons and tRNA anticodons pools, thus it might be reasonable to speculate a dynamic and regulated supply-and-demand relationship between translation and localized cellular translational machineries, such as tRNA pools.
Third, the basal level of FcεRI protein synthesis was not maintained in cultured neutrophils, due to their well documented low transcriptional and translational capacity, thus preventing intracellular pool and cell surface replenishment.
We will refer to RE in pool 3 as translational efficiency of the gene (see Methods) following the definition in [ 17].
Insufficient tRNA pools can lead to translational stalling and premature termination.
This inverse relationship between co- and post-translational N-glycosylation likely arises from the availability of a larger pool of unglycosylated substrates available for post-translational N-glycosylation.
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