To determine the translation rate, cpm values were normalised with yeast growth (OD600).
These clusters can cause translational pausing, which reduces the local protein translation rate.
Given that these EOT loop positions are unchanged, and given that the post-translational dynamics is unaffected by the ribosomal translation rate, these results suggest that changing translation rate will not affect the final distribution of fully integrated topologies.
Therefore, these results suggest that MazF activity augmented the translation rate of mCherry-P.
O'Brien, E.P., Vendruscolo, M. & Dobson, C.M. Prediction of variable translation rate effects on cotranslational protein folding.
The large dataset allowed quantitation of global effects, such as translation rate on mRNA stability and mRNA secondary structure on translation rate.
However, translation rate from a successful preclinical trial to an approved drug is extremely low.
This explains why the translation rate is a key factor of modulating gene expressions in an operon.
Espah Borujeni, A., Channarasappa, A.S. & Salis, H.M. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites.
Using the RBS Calculator, a protein coding sequence's translation rate may be rationally controlled across a 100,000+ fold range.
All our results suggested that the high translation rate of DXS disturbs the refolding process of DXS.
