Exact(8)
The in vitro transcription and translation profile for JV was therefore studied in more detail.
The output of the algorithm was smoothed with a sliding window of 19 triplets (Figure S1) producing an average translation profile for each ORF.
Note that the stochastic appearance of local minima in the translation profile of random sequences with E. coli codon usage was 10.2 %.
The appearance, therefore, of a previously unseen 42 kDa protein in the in vitro transcription and translation profile was unexpected but this protein does represent a translation product for the JV 5'GS.
Only one global plant translation profile has been obtained from a mutant with a defect in the translation apparatus [ 20].
Both these motifs contain one isolated node, i.e. a cell type that is not connected by edges to any other cell types, indicating that this cell type is dissimilar based on the gene's expression or translation profile.
Similar(52)
The fraction of the highly abundant proteins is dominated by the ribosomal proteins (with an average size of 100 amino acids) whose translation profiles are fairly smooth.
We observed only a light increase in the statistical appearance of local minima in the translation profiles from 9.58%, 9.83% and 10.03% which is far below the observed for the E. coli genome (Figure 4).
All in all, translation profiling techniques can be helpful to uncover cancer cell behaviors specifically steered by disarranged translation, and therefore to suggest novel therapeutic targets.
The IPA program was used to gain insight into the potential functional implications of the spaceflight-induced changes in gene translation profiles in 48A9 cells.
Translation profiles derived from all constructs showed a low level of internal initiation (probably from Met88) within the upstream GFP gene.
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