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The mean distal translation observed was −0.15 mm for SmartSet HV and −0.16 mm for Palacos R.
We recognise that both models may be an oversimplification, particularly when considering the constraints of the soft tissue envelope on the angle of correction achievable and the actual translation observed at the osteotomy site (Table 2).
These results reflect that the differential enhancement on IRES-driven translatiobservedved in extracts treated with HIV-1 PR or MBP-2Apro was not due to changes in the stability of luc mRNAs.
Indeed, the residual translation observed in the absence of the fusA gene is targeted by antibiotics reported to alter spontaneous translocation artificially induced by treatment of ribosomes with dithiol-specific reagents [27].
Results show that the level of gM translation observed in samples spiked with the negative controls, bunyamwera virus nucleocapsid protein or GSTPYMΔC remained consistent, showing no increase in translation.
To test whether down regulation of translation observed in the myo1Δ strains was regulated by this mechanism, we analyzed eIF2p levels by Western blot analysis with antibodies specific for eIF2αp subunit phosphorylated on Ser-51 (eIF2α-P).
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In addition the genes in each group are functionally related (see Additional file 16), specifically, genes in G2 are mostly linked to translation and might be linked to the translation arrest observed during dormancy.
These experiments demonstrate that the global translation arrest observed after the induction of VSG221 RNAi was operating through a block in translation initiation rather than translation elongation.
Results show that PYM displayed a dose-dependent translation enhancement of luciferase; however, in contrast, no increase in translation was observed in samples spiked with increasing amounts of ORF57 protein.
In addition, a high degree of uncoupling between transcription and translation was observed when investigating correlations in co-expression and co-translation relationships.
Given these observations, we attempted to rescue the cell size and translation defects observed in dsTOR-treated S2 cells by codepleting 4EBP with dsRNA.
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