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The translation index for each reporter was calculated as the ratio of changes in luciferase activity (RL/FL) relative to the ratio of changes in luciferase mRNA levels (RL mRNA /FL mRNA).
The reporter bearing CSF2 3'UTR also showed an elevated translation index, despite not being a direct target of NF90 (Fig. 2C,D), suggesting that the effect of NF90 on the CSF2 3'UTR was indirect.
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In WI-38 cells, high translation indexes were seen for reporters expressing the 3'UTRs of CXCL1, IL8, IL6, and CCL16 mRNAs, further suggesting that NF90 repressed their translation.
Figure 1 shows the multi-step CWT magnitude of 64-QAM signal and the constant CWT magnitude of normalized 64-QAM signal (as a function of the translation sampling index).
The suggested translation efficiency index is based on the analysis of the organism's ribosome profiling data.
CAI by Kudla et al. (2009) and translation adaptation index (tAI) by Tuller et al. (2010) as proxies for translation elongation efficiency are both problematic and can lead to serious bias, as will be illustrated in the next section.
Furthermore, to evaluate the suitability of any exogenous coding sequence to be efficiently expressed in A. gambiae, we generated a Perl script (File S4) that attributes a "translation penalty index" for any input coding sequence.
For some of the analyzed organisms, a decrease in correlation between the different translation efficiency indexes and protein abundance was observed when controlling for mRNA levels.
Then, we checked the 95% confidence intervals of each one of the translation efficiency indexes and found that they do not include the value 0 for the variable MTDR (see the section Materials and Methods), demonstrating that the MTDR index contributes additional information to protein abundance prediction given the other two indexes.
Multi-layer-index update block updates the translation table, label-index tables, and data tables, and maintains the correctness of index results in each layer.
After minor modifications, the back translation of the index was verified by the original authors of the Child-OIDP index at the University College London, UK.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com